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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NB100-1263 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1263, RRID:AB_2104890
- Product name
- Goat Polyclonal HNF-3 beta/FoxA2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoforms.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Nuclear pregnane X receptor cross-talk with FoxA2 to mediate drug-induced regulation of lipid metabolism in fasting mouse liver.
Foxa2 (HNF3beta ) controls multiple genes implicated in metabolism-secretion coupling of glucose-induced insulin release.
Nakamura K, Moore R, Negishi M, Sueyoshi T
The Journal of biological chemistry 2007 Mar 30;282(13):9768-76
The Journal of biological chemistry 2007 Mar 30;282(13):9768-76
Foxa2 (HNF3beta ) controls multiple genes implicated in metabolism-secretion coupling of glucose-induced insulin release.
Wang H, Gauthier BR, Hagenfeldt-Johansson KA, Iezzi M, Wollheim CB
The Journal of biological chemistry 2002 May 17;277(20):17564-70
The Journal of biological chemistry 2002 May 17;277(20):17564-70
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (1ug/ml) staining of Rat Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
