PA5-47001

antibody from Invitrogen Antibodies

Targeting: SOX10 DOM, WS2E, WS4

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47001

Provider

Invitrogen Antibodies

Product name

SOX10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) SOX2, rhSOX7 and rhSOX17 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Migration and fate of vestibular melanocytes during the development of the human inner ear.

van Beelen ESA, van der Valk WH, de Groot JCMJ, Hensen EF, Locher H, van Benthem PPG
Developmental neurobiology 2020 Nov;80(11-12):411-432

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Goat Anti-human SOX10 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47001) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to nuclei.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SOX10 was performed using 70% confluent log phase A-375 cells treated with SOX10 polyclonal antibody (Product # PA5-47001). The cells were fixed with 4% paraformaldehyde for 10 Minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. cells were labeled with SOX10 polyclonal antibody (Product # PA5-47001) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27012) at a dilution of 1:2,000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) assay of endogenous SOX10 protein using Anti-SOX10 Antibody: ChIP was performed using Anti-SOX10 Goat Polyclonal Antibody (Product # PA5-47001, 5 µg) on sheared chromatin from A-375 cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Goat IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to MYRF promoter, MIA, MPZ and SH3TC2 transcriptional start sites and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.