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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- PA5-47001 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) SOX2, rhSOX7 and rhSOX17 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Migration and fate of vestibular melanocytes during the development of the human inner ear.
van Beelen ESA, van der Valk WH, de Groot JCMJ, Hensen EF, Locher H, van Benthem PPG
Developmental neurobiology 2020 Nov;80(11-12):411-432
Developmental neurobiology 2020 Nov;80(11-12):411-432
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SOX10 was achieved by transfecting A-375 with SOX10 specific siRNAs (Silencer® select Product # s13311, s13309). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the SOX10 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SOX10 Polyclonal Antibody (Product # PA5-47001, 0.5ug/ml) and Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SOX10.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SOX10 Polyclonal Antibody (Product # PA5-47001) and 58, 62kDa bands corresponding to SOX10 was observed across cell lines tested and the protein expression was low in SW480. Modified whole cell extracts (1% SDS) (30 µg lysate) of A-375 (Lane 1), B16-F10 (Lane 2) and SW480 (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5ug/ml) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Goat Anti-human SOX10 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47001) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to nuclei.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SOX10 was performed using 70% confluent log phase A-375 cells treated with SOX10 polyclonal antibody (Product # PA5-47001). The cells were fixed with 4% paraformaldehyde for 10 Minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. cells were labeled with SOX10 polyclonal antibody (Product # PA5-47001) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27012) at a dilution of 1:2,000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay of endogenous SOX10 protein using Anti-SOX10 Antibody: ChIP was performed using Anti-SOX10 Goat Polyclonal Antibody (Product # PA5-47001, 5 µg) on sheared chromatin from A-375 cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Goat IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to MYRF promoter, MIA, MPZ and SH3TC2 transcriptional start sites and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
