Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [7]
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- Product number
- NBP2-59050 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal SOX10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human SOX10 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Feline
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing trigeminal ganglion and ear placode.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing dorsal root ganglia.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cerebral cortex shows strong nuclear immunoreactivity in a subset of glial cells.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human breast shows strong nuclear positivity in glandular cells.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cervix shows nuclear immunoreactivity in a subset of basal cells in squamous epithelium.
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human pancreas shows absence of positivity as expected (negative control).
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Frozen: SOX10 Antibody (CL4455) [NBP2-59050] - Feline adult spinal cord demonstrating SOX10 (red) and DAPI (blue). Antibody at 1:200 dilutiion in 1X PBS + 0.05% tween 20+ 2% BSA. Tested at 1:50, 1:100, 1:200, 1:500, and 1:1000. Dilution of 1:200 gave the best signal/noise ratio for IHC-Fr. The slides were subsequently incubated with Alexa Fluor conjugated secondary antibody for 1hr at RT and imaged with fluorescent microscopy. Image from verified customer review.