Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-76378 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SOX10 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 2 - 8 C 1 year from date of receipt
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOX10 Antibody [NBP2-76378] - Samples: Whole cell lysate (15 ug) from Malme-3M, Hep-G2, MDA-MB-435, HEK293T, SK-MEL-28, HeLa, UACC-257, Jurkat, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SOX10 antibody NBP2-76378 used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SOX10 Antibody [NBP2-76378] - Detection of human SOX10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Malme-3M cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SOX10 antibody NBP2-76378 used for IP at 100 ul per reaction. SOX10 was also immunoprecipitated by two other rabbit anti-SOX10 antibodies. For blotting immunoprecipitated SOX10,NBP2-76378 was used at 1:400. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SOX10 Antibody [NBP2-76378] - Detection of human SOX10 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-SOX10 antibody NBP2-76378 used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.