LS-C763307

antibody from LSBio

Targeting: SOX10 DOM, WS2E, WS4

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C763307

Provider

LSBio

Product name

SOX10 Antibody (aa416-466) LS-C763307

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 year.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human SOX10 by western blot. Samples: Whole cell lysate (15 µg) from Malme-3M, Hep-G2, MDA-MB-435, HEK293T, SK-MEL-28, HeLa, UACC-257, Jurkat, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SOX10 antibody used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH (A300-639A).

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human SOX10 by immunocytochemistry. Sample: FFPE section of UACC-257 cells. Antibody: Rabbit anti-SOX10 antibody (A305-799A-M lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human SOX10 by immunocytochemistry. Sample: FFPE section of Malme-3M cells. Antibody: Rabbit anti-SOX10 antibody (A305-799A-M lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of human SOX10 by immunocytochemistry. Sample: FFPE section of UACC-257 cells. Antibody: Rabbit anti-SOX10 antibody (A305-799A-M lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of human SOX10 by immunocytochemistry. Sample: FFPE section of Malme-3M cells. Antibody: Rabbit anti-SOX10 antibody (A305-799A-M lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human SOX10 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-SOX10 antibody (A305-799A-M lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human SOX10 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-SOX10 antibody (A305-799A-M lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human SOX10 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-SOX10 antibody (A305-799A-M lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of human SOX10 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-SOX10 antibody (A305-799A-M lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of human SOX10 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-SOX10 antibody (A305-799A-M lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).