AP3337a

antibody from Abgent

Targeting: AKT2 PKBβ

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP3337a

Provider

Abgent

Proper citation

Abgent Cat#AP3337a, RRID:AB_889235

Product name

Phospho-AKT2(S474) Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Dot blot analysis of Phospho-AKT2-S474 polyclonal antibody (Cat# AP3337a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.

Primary Ab dilution

1:500