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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-37814 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is HepG2 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HepG2 cells, untreated or treated with EGF, using AKT1/AKT2/AKT3 (pTyr315/316/312) polyclonal antibody (Product # PA5-37814).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) in methanol-fixed Hela cells, using Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody (Product # PA5-37814).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) in paraffin-embedded Human breast carcinoma tissue using Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody (Product # PA5-37814) (left) or the same antibody preincubated with blocking peptide (right).
