TA319557

antibody from OriGene

Targeting: AKT2 PKBβ

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319557

Provider

OriGene

Product name

Rat monoclonal anti-AKT2 antibody

Antibody type

Monoclonal

Description

Rat monoclonal anti-AKT2 antibody

Host

Rat

Conjugate

Unconjugated

Epitope

AKT2

Antibody clone number

16G11.A7

Vial size

100 µg

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot of Rat anti-AKT2 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT2 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot of Rat Anti-AKT2 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 ug per lane. Primary antibody: AKT-2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Rat secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.

Validation comment

WB