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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 680202 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#680202, RRID:AB_2566344
- Product name
- Purified anti-AKT2
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant fragment (a.a. 2-149) of human AKT2 expressed in E. coli.
- Description
- AKT (Protein kinase B, PKB) is a serine/threonine kinase that plays a key in regulating cell survival, insulin signaling, angiogenesis, and tumor formation. There are three isoforms of AKT- AKT1, 2, and 3, which are also known as PKBα, β, and γ). Increasing evidence suggests that AKT1, 2, and 3 plays an unique role in breast cancer initiation and progression. AKT2 is amplified or overexpressed in cancer with a higher frequency than those found with AKT1. AKT2 is also closely linked to Type II diabetes and is a pivotal player in the Phosphatidylinositol 3 kinase/Akt/Mammalian target of rapamycin (PI3K/mTOR) signaling axis. The deregulation of AKT2 has been associated with acquisition of stem cell-like properties, responsible for invasiveness and tumor progression, metastatic spread, and chemoresistance.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- P81B6
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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- Experimental details
- Western blot analysis of cell lysates from HeLa (lane 1), Jurkat (lane 2), and NIH3T3 (lane 3). Purified anti-AKT2 antibody (clone P81B6 ) was used and then was followed by HRP anti-mouse IgG antibody. β-actin antibody (clone poly6221) was used as a loading control.
- Conjugate
- Unconjugated
