AM00109PU-N

antibody from OriGene

Targeting: AKT2 PKBβ

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AM00109PU-N

Provider

OriGene

Product name

AKT2 (specific) (incl. pos. control) mouse monoclonal antibody, clone 8B7, Purified

Antibody type

Monoclonal

Description

AKT2 (specific) (incl. pos. control) mouse monoclonal antibody, clone 8B7, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

AKT2

Isotype

IgG

Antibody clone number

8B7

Vial size

100 µg

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection of endogenous akt2 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab PKB-8B7 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2:SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF7 Lane 6: MDA-MB-231 Lane 7: T47D

Validation comment

WB