PAB0404

antibody from Abnova Corporation

Targeting: AKT2 PKBβ

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0404

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0404, RRID:AB_982311

Product name

AKT2 (phospho S474) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT2.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

A family with severe insulin resistance and diabetes due to a mutation in AKT2.

George S, Rochford JJ, Wolfrum C, Gray SL, Schinner S, Wilson JC, Soos MA, Murgatroyd PR, Williams RM, Acerini CL, Dunger DB, Barford D, Umpleby AM, Wareham NJ, Davies HA, Schafer AJ, Stoffel M, O'Rahilly S, Barroso I
Science (New York, N.Y.) 2004 May 28;304(5675):1325-8

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Protein kinase B inhibits apoptosis induced by actinomycin D in ECV304 cells through phosphorylation of caspase 8.

Shim D, Kang HY, Jeon BW, Kang SS, Chang SI, Kim HY
Archives of biochemistry and biophysics 2004 May 15;425(2):214-20

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Akt2 expression correlates with prognosis of human hepatocellular carcinoma.

Xu X, Sakon M, Nagano H, Hiraoka N, Yamamoto H, Hayashi N, Dono K, Nakamori S, Umeshita K, Ito Y, Matsuura N, Monden M
Oncology reports 2004 Jan;11(1):25-32

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of AKT2 (phospho S474) polyclonal antibody (Cat # PAB0404) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5 ug/mL. P-Pab : phospho-antibody; P-Peptide : phospho-peptide; NP-Peptide : non-phospho-peptide.

Validation comment

Dot Blot (Peptide)