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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Flow cytometry [2]
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- Product number
- TA500783 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500783, RRID:AB_11126999
- Product name
- AKT2 mouse monoclonal antibody, clone OTI8D9 (formerly 8D9)
- Antibody type
- Monoclonal
- Description
- AKT2 mouse monoclonal antibody, clone OTI8D9 (formerly 8D9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AKT2
- Isotype
- IgG
- Antibody clone number
- OTI8D9
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (RC217733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of AKT2 by using TrueMab monoclonal anti-AKT2 antibodies (Negative control: IP without adding anti-AKT2 antibody.). For each experiment, 500ul of DDK tagged AKT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-AKT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-AKT2 antibody(TA500783),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-AKT2 antibody(TA500783),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
