TA500831

antibody from OriGene

Targeting: AKT2 PKBβ

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500831

Provider

OriGene

Proper citation

OriGene Cat#TA500831, RRID:AB_11123975

Product name

AKT2 mouse monoclonal antibody, clone OTI4G1 (formerly 4G1)

Antibody type

Monoclonal

Description

AKT2 mouse monoclonal antibody, clone OTI4G1 (formerly 4G1)

Host

Mouse

Conjugate

Unconjugated

Epitope

AKT2

Isotype

IgG

Antibody clone number

OTI4G1

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (RC217733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.

Validation comment

WB

Immunoprecipitation

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunoprecipitation(IP) of AKT2 by using TrueMab monoclonal anti-AKT2 antibodies (Negative control: IP without adding anti-AKT2 antibody.). For each experiment, 500ul of DDK tagged AKT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-AKT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Validation comment

IP