ABIN362104

antibody from antibodies-online

Targeting: AKT2 PKBβ

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362104

Provider

antibodies-online

Product name

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (pSer474) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pSer474 (Q-F-S (p) -Y-S) derived from Human Akt2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

pSer474

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Phosphatidylinositol-3-OH Kinase (PI3K)/AKT2, activated in breast cancer, regulates and is induced by estrogen receptor alpha (ERalpha) via interaction between ERalpha and PI3K.

Sun M, Paciga JE, Feldman RI, Yuan Z, Coppola D, Lu YY, Shelley SA, Nicosia SV, Cheng JQ
Cancer research 2001 Aug 15;61(16):5985-91

---

Frequent activation of AKT2 and induction of apoptosis by inhibition of phosphoinositide-3-OH kinase/Akt pathway in human ovarian cancer.

Yuan ZQ, Sun M, Feldman RI, Wang G, Ma X, Jiang C, Coppola D, Nicosia SV, Cheng JQ
Oncogene 2000 May 4;19(19):2324-30

---

Mitogenic activation, phosphorylation, and nuclear translocation of protein kinase Bbeta.

Meier R, Alessi DR, Cron P, Andjelković M, Hemmings BA
The Journal of biological chemistry 1997 Nov 28;272(48):30491-7