Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN359078 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (N-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AKT2 Antibody.
- Description
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- N-Term
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references A family with severe insulin resistance and diabetes due to a mutation in AKT2.
Protein kinase B inhibits apoptosis induced by actinomycin D in ECV304 cells through phosphorylation of caspase 8.
Akt2, phosphatidylinositol 3-kinase, and PTEN are in lipid rafts of intestinal cells: role in absorption and differentiation.
Akt2 expression correlates with prognosis of human hepatocellular carcinoma.
Akt regulates basic helix-loop-helix transcription factor-coactivator complex formation and activity during neuronal differentiation.
George S, Rochford JJ, Wolfrum C, Gray SL, Schinner S, Wilson JC, Soos MA, Murgatroyd PR, Williams RM, Acerini CL, Dunger DB, Barford D, Umpleby AM, Wareham NJ, Davies HA, Schafer AJ, Stoffel M, O'Rahilly S, Barroso I
Science (New York, N.Y.) 2004 May 28;304(5675):1325-8
Science (New York, N.Y.) 2004 May 28;304(5675):1325-8
Protein kinase B inhibits apoptosis induced by actinomycin D in ECV304 cells through phosphorylation of caspase 8.
Shim D, Kang HY, Jeon BW, Kang SS, Chang SI, Kim HY
Archives of biochemistry and biophysics 2004 May 15;425(2):214-20
Archives of biochemistry and biophysics 2004 May 15;425(2):214-20
Akt2, phosphatidylinositol 3-kinase, and PTEN are in lipid rafts of intestinal cells: role in absorption and differentiation.
Li X, Leu S, Cheong A, Zhang H, Baibakov B, Shih C, Birnbaum MJ, Donowitz M
Gastroenterology 2004 Jan;126(1):122-35
Gastroenterology 2004 Jan;126(1):122-35
Akt2 expression correlates with prognosis of human hepatocellular carcinoma.
Xu X, Sakon M, Nagano H, Hiraoka N, Yamamoto H, Hayashi N, Dono K, Nakamori S, Umeshita K, Ito Y, Matsuura N, Monden M
Oncology reports 2004 Jan;11(1):25-32
Oncology reports 2004 Jan;11(1):25-32
Akt regulates basic helix-loop-helix transcription factor-coactivator complex formation and activity during neuronal differentiation.
Vojtek AB, Taylor J, DeRuiter SL, Yu JY, Figueroa C, Kwok RP, Turner DL
Molecular and cellular biology 2003 Jul;23(13):4417-27
Molecular and cellular biology 2003 Jul;23(13):4417-27
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- (LEFT)Western blot analysis of anti-AKT2 Antibody (N-term) Pab in mouse liver tissue lysates (35ug/lane). AKT2(arrow) was detected using the purified Pab (1:60 dilution). (RIGHT)Western blot analysis of anti-AKT2 Antibody (N-term) Pab in 293 cell line lysates transiently transfected with the AKT2 gene (2ug/lane). AKT2(arrow) was detected using the purified Pab (1:60 dilution).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma reacted with AKT2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.