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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-56676 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56676, RRID:AB_837545
- Product name
- Mouse Monoclonal AKT2 Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. AKT2 (95C657.1.2)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 0.2 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT2 Antibody (95C657.1.2) [NB100-56676] - analysis of Akt2 using this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: AKT2 Antibody (95C657.1.2) [NB100-56676] - An intracellular stain was performed on A549 cells with AKT2 Antibody (95C657.1.2) NB100-56676 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgM (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488.
