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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-81780 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-81780, RRID:AB_1144423
- Product name
- Rabbit Polyclonal AKT2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Akt2 (specific to Phospho-Ser474) detects endogenous levels of Akt2 only when phosphorylated at serine 474.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Human NUMB6 Induces Epithelial-Mesenchymal Transition and Enhances Breast Cancer Cells Migration and Invasion.
Karaczyn AA, Adams TL, Cheng RY, Matluk NN, Verdi JM
Journal of cellular biochemistry 2017 Feb;118(2):237-251
Journal of cellular biochemistry 2017 Feb;118(2):237-251
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT2 [p Ser474] Antibody [NB100-81780] - Analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using Akt2(Phospho-Ser474) antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Akt2 [p Ser474] Antibody [NB100-81780] - Analysis of extracts from 293 cells, treated with serum or calf intestinal phosphatase (CIP), using Akt2 (Phospho-Ser474) Antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: AKT2 [p Ser474] Antibody [NB100-81780] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2 (phospho- Ser474) antibody
