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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-39778 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Bax (Ser184) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Reduced Mitochondrial Apoptotic Priming Drives Resistance to BH3 Mimetics in Acute Myeloid Leukemia.
Metformin Inhibit Lung Cancer Cell Growth and Invasion in Vitro as Well as Tumor Formation in Vivo Partially by Activating PP2A.
Bhatt S, Pioso MS, Olesinski EA, Yilma B, Ryan JA, Mashaka T, Leutz B, Adamia S, Zhu H, Kuang Y, Mogili A, Louissaint A Jr, Bohl SR, Kim AS, Mehta AK, Sanghavi S, Wang Y, Morris E, Halilovic E, Paweletz CP, Weinstock DM, Garcia JS, Letai A
Cancer cell 2020 Dec 14;38(6):872-890.e6
Cancer cell 2020 Dec 14;38(6):872-890.e6
Metformin Inhibit Lung Cancer Cell Growth and Invasion in Vitro as Well as Tumor Formation in Vivo Partially by Activating PP2A.
Zhou X, Liu S, Lin X, Xu L, Mao X, Liu J, Zhang Z, Jiang W, Zhou H
Medical science monitor : international medical journal of experimental and clinical research 2019 Jan 29;25:836-846
Medical science monitor : international medical journal of experimental and clinical research 2019 Jan 29;25:836-846
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Bax pSer184 in extracts from K562 cells using a Phospho-Bax pSer184 polyclonal antibody (Product # PA5-39778).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of A549 (Lane 1) and A549 treated with Etoposide (50 uM, 16 hour) (Lane 2). The blot was probed with Anti-Phospho-Bax (Ser184) Polyclonal Antibody (Product # PA5-39778, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 18 kDa band corresponding to Bax was observed in A549 and was enhanced upon cell treatment.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-Bax pSer184 in paraffin-embedded human brain tissue using a Phospho-Bax pSer184 polyclonal antibody (Product # PA5-39778).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA using a Phospho-Bax pSer184 polyclonal antibody (Product # PA5-39778) comparing an epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings were taken at 450nM.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Western blot detecting Bax, Bcl-2, Myc, and Akt protein expression as well as serine phosphorylation of Bax, Myc, and Akt at indicated sites in A549 cells. ( A-D ) Bax and Bcl-2 protein expression as well as serine phosphorylation of Bax in A549 cells. ( G-K ) Myc and Akt protein expression as well as serine phosphorylation of Myc and Akt in A549 cells. ( E, F, L ) Representative western blot results. Data in other panels was normalized to the mean value of NC group in the same panel. ns, non-significant; * P
