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antibody from NSJ Bioreagents
Targeting: BAX
BCL2L4
Western blot
ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- V2005 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Bax Antibody
- Antibody type
- Monoclonal
- Antigen
- Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody.
- Description
- Protein G purified antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 2D2
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Bax antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Primary multiple tumor with affection of the thyroid gland, uterus, urinary bladder, mammary gland and other organs
Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E2 in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell Apoptosis.
Characterizing Bcl-2 Family Protein Conformation and Oligomerization Using Cross-Linking and Antibody Gel-Shift in Conjunction with Native PAGE.
Anti- and Pro-apoptotic Bcl2 Proteins Distribution and Metabolic Profile in Human Coronary Aorta Endothelial Cells Before and After HypPDT.
Suppression of c-Myc induces apoptosis via an AMPK/mTOR-dependent pathway by 4-O-methyl-ascochlorin in leukemia cells.
Maspin-related Orchestration of Aggressiveness of Gastric Cancer.
STAT3 and NF-κB cooperatively control in vitro spontaneous apoptosis and poor chemo-responsiveness in patients with chronic lymphocytic leukemia.
Nedaplatin sensitization of cisplatin-resistant human non-small cell lung cancer cells.
Dynamin-related protein Drp1 is required for Bax translocation to mitochondria in response to irradiation-induced apoptosis.
In non-transformed cells Bak activates upon loss of anti-apoptotic Bcl-XL and Mcl-1 but in the absence of active BH3-only proteins.
The overexpression and nuclear translocation of Trx-1 during hypoxia confers on HepG2 cells resistance to DDP, and GL-V9 reverses the resistance by suppressing the Trx-1/Ref-1 axis.
Gastric cancer in young vs old Romanian patients: immunoprofile with emphasis on maspin and mena protein reactivity.
Oxazoloisoindolinones with in vitro antitumor activity selectively activate a p53-pathway through potential inhibition of the p53-MDM2 interaction.
Enhanced cytotoxicity of prenylated chalcone against tumour cells via disruption of the p53-MDM2 interaction.
Mcl-1 downregulation leads to the heightened sensitivity exhibited by BCR-ABL positive ALL to induction of energy and ER-stress.
Role of JNK Activation and Mitochondrial Bax Translocation in Allicin-Induced Apoptosis in Human Ovarian Cancer SKOV3 Cells.
Pro-oxidative action of polyphenols as action mechanism for their pro-apoptotic activity.
Inhibition of apoptotic Bax translocation to the mitochondria is a central function of parkin.
The novel PI3 kinase inhibitor, BAY 80-6946, impairs melanoma growth in vivo and in vitro.
Romaniuk А, Lyndin M, Smiyanov V, Sikora V, Rieznik A, Kuzenko Y, Budko H, Moskalenko Y, Karpenko L, Sikora V, Gladchenko O
Pathology - Research and Practice 2017 January
Pathology - Research and Practice 2017 January
Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E2 in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell Apoptosis.
Li Y, Ma H, Lu Y, Tan BJ, Xu L, Lawal TO, Mahady GB, Liu D
BioMed research international 2016;2016:2574637
BioMed research international 2016;2016:2574637
Characterizing Bcl-2 Family Protein Conformation and Oligomerization Using Cross-Linking and Antibody Gel-Shift in Conjunction with Native PAGE.
Dewson G
Methods in molecular biology (Clifton, N.J.) 2016;1419:185-96
Methods in molecular biology (Clifton, N.J.) 2016;1419:185-96
Anti- and Pro-apoptotic Bcl2 Proteins Distribution and Metabolic Profile in Human Coronary Aorta Endothelial Cells Before and After HypPDT.
Maslaňáková M, Balogová L, Miškovský P, Tkáčová R, Štroffeková K
Cell biochemistry and biophysics 2016 Sep;74(3):435-47
Cell biochemistry and biophysics 2016 Sep;74(3):435-47
Suppression of c-Myc induces apoptosis via an AMPK/mTOR-dependent pathway by 4-O-methyl-ascochlorin in leukemia cells.
Shin JM, Jeong YJ, Cho HJ, Magae J, Bae YS, Chang YC
Apoptosis : an international journal on programmed cell death 2016 May;21(5):657-68
Apoptosis : an international journal on programmed cell death 2016 May;21(5):657-68
Maspin-related Orchestration of Aggressiveness of Gastric Cancer.
Gurzu S, Kadar Z, Sugimura H, Orlowska J, Bara T, Bara T Jr, Szederjesi J, Jung I
Applied immunohistochemistry & molecular morphology : AIMM 2016 May-Jun;24(5):326-36
Applied immunohistochemistry & molecular morphology : AIMM 2016 May-Jun;24(5):326-36
STAT3 and NF-κB cooperatively control in vitro spontaneous apoptosis and poor chemo-responsiveness in patients with chronic lymphocytic leukemia.
Liu FT, Jia L, Wang P, Wang H, Farren TW, Agrawal SG
Oncotarget 2016 May 31;7(22):32031-45
Oncotarget 2016 May 31;7(22):32031-45
Nedaplatin sensitization of cisplatin-resistant human non-small cell lung cancer cells.
Wang H, Zhu X, Huang J, Chen P, Han S, Yan X
Oncology letters 2016 Apr;11(4):2566-2572
Oncology letters 2016 Apr;11(4):2566-2572
Dynamin-related protein Drp1 is required for Bax translocation to mitochondria in response to irradiation-induced apoptosis.
Wang P, Wang P, Liu B, Zhao J, Pang Q, Agrawal SG, Jia L, Liu FT
Oncotarget 2015 Sep 8;6(26):22598-612
Oncotarget 2015 Sep 8;6(26):22598-612
In non-transformed cells Bak activates upon loss of anti-apoptotic Bcl-XL and Mcl-1 but in the absence of active BH3-only proteins.
Senft D, Weber A, Saathoff F, Berking C, Heppt MV, Kammerbauer C, Rothenfusser S, Kellner S, Kurgyis Z, Besch R, Häcker G
Cell death & disease 2015 Nov 26;6:e1996
Cell death & disease 2015 Nov 26;6:e1996
The overexpression and nuclear translocation of Trx-1 during hypoxia confers on HepG2 cells resistance to DDP, and GL-V9 reverses the resistance by suppressing the Trx-1/Ref-1 axis.
Zhao L, Li W, Zhou Y, Zhang Y, Huang S, Xu X, Li Z, Guo Q
Free radical biology & medicine 2015 May;82:29-41
Free radical biology & medicine 2015 May;82:29-41
Gastric cancer in young vs old Romanian patients: immunoprofile with emphasis on maspin and mena protein reactivity.
Gurzu S, Kadar Z, Sugimura H, Bara T, Bara T Jr, Halmaciu I, Jung I
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2015 Mar;123(3):223-33
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2015 Mar;123(3):223-33
Oxazoloisoindolinones with in vitro antitumor activity selectively activate a p53-pathway through potential inhibition of the p53-MDM2 interaction.
Soares J, Pereira NA, Monteiro Â, Leão M, Bessa C, Dos Santos DJ, Raimundo L, Queiroz G, Bisio A, Inga A, Pereira C, Santos MM, Saraiva L
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2015 Jan 23;66:138-47
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2015 Jan 23;66:138-47
Enhanced cytotoxicity of prenylated chalcone against tumour cells via disruption of the p53-MDM2 interaction.
Leão M, Soares J, Gomes S, Raimundo L, Ramos H, Bessa C, Queiroz G, Domingos S, Pinto M, Inga A, Cidade H, Saraiva L
Life sciences 2015 Dec 1;142:60-5
Life sciences 2015 Dec 1;142:60-5
Mcl-1 downregulation leads to the heightened sensitivity exhibited by BCR-ABL positive ALL to induction of energy and ER-stress.
Leclerc GJ, DeSalvo J, Du J, Gao N, Leclerc GM, Lehrman MA, Lampidis TJ, Barredo JC
Leukemia research 2015 Aug 20;
Leukemia research 2015 Aug 20;
Role of JNK Activation and Mitochondrial Bax Translocation in Allicin-Induced Apoptosis in Human Ovarian Cancer SKOV3 Cells.
Xu L, Yu J, Zhai D, Zhang D, Shen W, Bai L, Cai Z, Yu C
Evidence-based complementary and alternative medicine : eCAM 2014;2014:378684
Evidence-based complementary and alternative medicine : eCAM 2014;2014:378684
Pro-oxidative action of polyphenols as action mechanism for their pro-apoptotic activity.
Lecci RM, Logrieco A, Leone A
Anti-cancer agents in medicinal chemistry 2014;14(10):1363-75
Anti-cancer agents in medicinal chemistry 2014;14(10):1363-75
Inhibition of apoptotic Bax translocation to the mitochondria is a central function of parkin.
Charan RA, Johnson BN, Zaganelli S, Nardozzi JD, LaVoie MJ
Cell death & disease 2014 Jul 3;5:e1313
Cell death & disease 2014 Jul 3;5:e1313
The novel PI3 kinase inhibitor, BAY 80-6946, impairs melanoma growth in vivo and in vitro.
Schneider P, Schön M, Pletz N, Seitz CS, Liu N, Ziegelbauer K, Zachmann K, Emmert S, Schön MP
Experimental dermatology 2014 Aug;23(8):579-84
Experimental dermatology 2014 Aug;23(8):579-84
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A549 cell lysate with Bax antibody (clone 2D2). Predicted molecular weight ~21 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed human Jurkat cells with Bax antibody (clone 2D2, green) and NucSpot nuclear stain (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE Hodgkin's lymphoma stained with Bax antibody (clone 2D2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed human Jurkat cells with Bax antibody (clone 2D2); Gold=isotype control, Blue= Bax antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free Bax antibody (clone 2D2) as confirmation of integrity and purity.
