Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-44418 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Bax Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 7 ml
- Storage
- Store at 4C.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: Bax Antibody (2D2) - IHC-Prediluted [NBP2-44418] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with Bax 2D2 antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Perform antigen retrieval by boiling tissue sections in 10mM citrate buffer, pH 6.0. Incubate the 2D2 antibody for 30 min at RT.