Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations [0]
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- Product number
- 14-6997-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Bax Monoclonal Antibody (6A7), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 6A7 antibody reacts with mouse, rat, monkey and human Bax (20 kDa). The 6A7 antibody shows no cross-reaction with Bcl-2 or Bcl-xL proteins. Bcl-2, Bax, and Bcl-xL are members of a protein family that regulates apoptosis; while several family members including Bcl-2 and Bcl-xL inhibit apoptosis, overexpression of Bax promotes programmed cell death. A human Bax protein peptide sequence, aa 12-24, was used as immunogen. Applications Reported: The 6A7 antibody has been reported for use in immunoblotting (WB). Applications Tested: The 6A7 antibody has been tested by Western Blot and can be used at approximately 1 µg/mL. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6A7
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Removal of syndecan-1 promotes TRAIL-induced apoptosis in myeloma cells.
Identification of Bax-voltage-dependent anion channel 1 complexes in digitonin-solubilized cerebellar granule neurons.
Dampening of death pathways by schnurri-2 is essential for T-cell development.
RUNX3 modulates DNA damage-mediated phosphorylation of tumor suppressor p53 at Ser-15 and acts as a co-activator for p53.
Stabilization of p73 by nuclear IkappaB kinase-alpha mediates cisplatin-induced apoptosis.
Caspase inhibition blocks cell death and results in cell cycle arrest in cytokine-deprived hematopoietic cells.
Taurine monochloramine activates a cell death pathway involving Bax and Caspase-9.
Nonionic detergents induce dimerization among members of the Bcl-2 family.
Wu YH, Yang CY, Chien WL, Lin KI, Lai MZ
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 15;188(6):2914-21
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 15;188(6):2914-21
Identification of Bax-voltage-dependent anion channel 1 complexes in digitonin-solubilized cerebellar granule neurons.
Huckabee DB, Jekabsons MB
Journal of neurochemistry 2011 Dec;119(5):1137-50
Journal of neurochemistry 2011 Dec;119(5):1137-50
Dampening of death pathways by schnurri-2 is essential for T-cell development.
Staton TL, Lazarevic V, Jones DC, Lanser AJ, Takagi T, Ishii S, Glimcher LH
Nature 2011 Apr 7;472(7341):105-9
Nature 2011 Apr 7;472(7341):105-9
RUNX3 modulates DNA damage-mediated phosphorylation of tumor suppressor p53 at Ser-15 and acts as a co-activator for p53.
Yamada C, Ozaki T, Ando K, Suenaga Y, Inoue K, Ito Y, Okoshi R, Kageyama H, Kimura H, Miyazaki M, Nakagawara A
The Journal of biological chemistry 2010 May 28;285(22):16693-703
The Journal of biological chemistry 2010 May 28;285(22):16693-703
Stabilization of p73 by nuclear IkappaB kinase-alpha mediates cisplatin-induced apoptosis.
Furuya K, Ozaki T, Hanamoto T, Hosoda M, Hayashi S, Barker PA, Takano K, Matsumoto M, Nakagawara A
The Journal of biological chemistry 2007 Jun 22;282(25):18365-78
The Journal of biological chemistry 2007 Jun 22;282(25):18365-78
Caspase inhibition blocks cell death and results in cell cycle arrest in cytokine-deprived hematopoietic cells.
Brown NM, Martin SM, Maurice N, Kuwana T, Knudson CM
The Journal of biological chemistry 2007 Jan 26;282(4):2144-55
The Journal of biological chemistry 2007 Jan 26;282(4):2144-55
Taurine monochloramine activates a cell death pathway involving Bax and Caspase-9.
Emerson DK, McCormick ML, Schmidt JA, Knudson CM
The Journal of biological chemistry 2005 Feb 4;280(5):3233-41
The Journal of biological chemistry 2005 Feb 4;280(5):3233-41
Nonionic detergents induce dimerization among members of the Bcl-2 family.
Hsu YT, Youle RJ
The Journal of biological chemistry 1997 May 23;272(21):13829-34
The Journal of biological chemistry 1997 May 23;272(21):13829-34
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