Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [2]
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Validation data
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- Product number
- 14-6997-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bax Monoclonal Antibody (6A7), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 6A7 antibody reacts with mouse, rat, monkey and human Bax (20 kDa). The 6A7 antibody shows no cross-reaction with Bcl-2 or Bcl-xL proteins. Bcl-2, Bax, and Bcl-xL are members of a protein family that regulates apoptosis; while several family members including Bcl-2 and Bcl-xL inhibit apoptosis, overexpression of Bax promotes programmed cell death. A human Bax protein peptide sequence, aa 12-24, was used as immunogen. Applications Reported: The 6A7 antibody has been reported for use in immunoblotting (WB). Applications Tested: The 6A7 antibody has been tested by Western Blot and can be used at approximately 1 µg/mL. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6A7
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Multinucleated polyploid cardiomyocytes undergo an enhanced adaptability to hypoxia via mitophagy.
The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death.
Mammalian STE20-Like Kinase 1 Deletion Alleviates Renal Ischaemia-Reperfusion Injury via Modulating Mitophagy and the AMPK-YAP Signalling Pathway.
Therapeutic effect of Sirtuin 3 on ameliorating nonalcoholic fatty liver disease: The role of the ERK-CREB pathway and Bnip3-mediated mitophagy.
Removal of syndecan-1 promotes TRAIL-induced apoptosis in myeloma cells.
Identification of Bax-voltage-dependent anion channel 1 complexes in digitonin-solubilized cerebellar granule neurons.
Dampening of death pathways by schnurri-2 is essential for T-cell development.
RUNX3 modulates DNA damage-mediated phosphorylation of tumor suppressor p53 at Ser-15 and acts as a co-activator for p53.
Stabilization of p73 by nuclear IkappaB kinase-alpha mediates cisplatin-induced apoptosis.
Caspase inhibition blocks cell death and results in cell cycle arrest in cytokine-deprived hematopoietic cells.
Taurine monochloramine activates a cell death pathway involving Bax and Caspase-9.
Nonionic detergents induce dimerization among members of the Bcl-2 family.
Jiang YH, Wang HL, Peng J, Zhu Y, Zhang HG, Tang FQ, Jian Z, Xiao YB
Journal of molecular and cellular cardiology 2020 Jan;138:115-135
Journal of molecular and cellular cardiology 2020 Jan;138:115-135
The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death.
Zierhut C, Yamaguchi N, Paredes M, Luo JD, Carroll T, Funabiki H
Cell 2019 Jul 11;178(2):302-315.e23
Cell 2019 Jul 11;178(2):302-315.e23
Mammalian STE20-Like Kinase 1 Deletion Alleviates Renal Ischaemia-Reperfusion Injury via Modulating Mitophagy and the AMPK-YAP Signalling Pathway.
Feng J, Li H, Zhang Y, Wang Q, Zhao S, Meng P, Li J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;51(5):2359-2376
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;51(5):2359-2376
Therapeutic effect of Sirtuin 3 on ameliorating nonalcoholic fatty liver disease: The role of the ERK-CREB pathway and Bnip3-mediated mitophagy.
Li R, Xin T, Li D, Wang C, Zhu H, Zhou H
Redox biology 2018 Sep;18:229-243
Redox biology 2018 Sep;18:229-243
Removal of syndecan-1 promotes TRAIL-induced apoptosis in myeloma cells.
Wu YH, Yang CY, Chien WL, Lin KI, Lai MZ
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 15;188(6):2914-21
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 15;188(6):2914-21
Identification of Bax-voltage-dependent anion channel 1 complexes in digitonin-solubilized cerebellar granule neurons.
Huckabee DB, Jekabsons MB
Journal of neurochemistry 2011 Dec;119(5):1137-50
Journal of neurochemistry 2011 Dec;119(5):1137-50
Dampening of death pathways by schnurri-2 is essential for T-cell development.
Staton TL, Lazarevic V, Jones DC, Lanser AJ, Takagi T, Ishii S, Glimcher LH
Nature 2011 Apr 7;472(7341):105-9
Nature 2011 Apr 7;472(7341):105-9
RUNX3 modulates DNA damage-mediated phosphorylation of tumor suppressor p53 at Ser-15 and acts as a co-activator for p53.
Yamada C, Ozaki T, Ando K, Suenaga Y, Inoue K, Ito Y, Okoshi R, Kageyama H, Kimura H, Miyazaki M, Nakagawara A
The Journal of biological chemistry 2010 May 28;285(22):16693-703
The Journal of biological chemistry 2010 May 28;285(22):16693-703
Stabilization of p73 by nuclear IkappaB kinase-alpha mediates cisplatin-induced apoptosis.
Furuya K, Ozaki T, Hanamoto T, Hosoda M, Hayashi S, Barker PA, Takano K, Matsumoto M, Nakagawara A
The Journal of biological chemistry 2007 Jun 22;282(25):18365-18378
The Journal of biological chemistry 2007 Jun 22;282(25):18365-18378
Caspase inhibition blocks cell death and results in cell cycle arrest in cytokine-deprived hematopoietic cells.
Brown NM, Martin SM, Maurice N, Kuwana T, Knudson CM
The Journal of biological chemistry 2007 Jan 26;282(4):2144-55
The Journal of biological chemistry 2007 Jan 26;282(4):2144-55
Taurine monochloramine activates a cell death pathway involving Bax and Caspase-9.
Emerson DK, McCormick ML, Schmidt JA, Knudson CM
The Journal of biological chemistry 2005 Feb 4;280(5):3233-41
The Journal of biological chemistry 2005 Feb 4;280(5):3233-41
Nonionic detergents induce dimerization among members of the Bcl-2 family.
Hsu YT, Youle RJ
The Journal of biological chemistry 1997 May 23;272(21):13829-34
The Journal of biological chemistry 1997 May 23;272(21):13829-34
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of BAX was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR612938_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of BAX was performed by loading 50 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa BAX KO (Lane 3) whole cell extracts. The samples were electrophoresed using Novex™ 16%, Tricine, 1.0 mm, Mini Protein Gel (Product # EC66952BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-Bax Monoclonal Antibody (6A7), eBioscience™ (Product # 14-6997-82, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:5,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to BAX.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Bax Monoclonal Antibody (6A7), eBioscience™(Product # 14-6997-82) and a 19kDa band corresponding to Bax was observed across cell lines tested. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MCF7 treated with etoposide (50uM,16 Hrs.) (Lane 2), A549 (Lane 3), A549 treated with etoposide (50uM,16 Hrs.) (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Expression of BAX was found to be up regulated upon etoposide treatment in A549 and MCF-7 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Sirt3 sustains mitochondrial function and blocks mitochondrial apoptosis activation. A. The mitochondrial membrane potential was evaluated using TRME. The relative TMRE fluorescence was evaluated using spectrophotometer. B-E. After PA treatment, hepatocytes were collected, and the proteins were isolated. Then, western blotting was performed to analyse the expression of the mitochondrial respiratory complex. Tom20 was the loading control for the mitochondrial proteins. F-G. Immunofluorescence assay for cyt-c liberation. DAPI was used to tag the nucleus. The fusion of cyt-c and DAPI indicates the leakage of cyt-c from mitochondria into the cytoplasm/nucleus. H-M. The anti-apoptotic and pro-apoptotic protein expression was detected to quantify the mitochondrial apoptosis using western blotting analysis. The data represent the mean +- SEM (n = 4 mice per group). *P < 0.05. Fig. 4