M00183-1

antibody from Boster Biological Technology

Targeting: BAX BCL2L4

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00183-1

Provider

Boster Biological Technology

Product name

Anti-Bax Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for BAX detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IHC, IP, IHC, WB. Reactive species: Human, Mouse, Rat BAX information: Molecular Weight: 21184 MW; Subcellular Localization: Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Colocalizes with 14- 3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane; Tissue Specificity: Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

AAB-2

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

The protective effect of Macrostemonoside T from Allium macrostemon Bunge against Isoproterenol-Induced myocardial injury via the PI3K/Akt/mTOR signaling pathway.

Wu J, Cui Y, Ding W, Zhang J, Wang L
International immunopharmacology 2024 May 30;133:112086

---

Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability.

Chen F, Yu X
Cardiovascular toxicology 2024 Jul;24(7):625-636

---

Cytisine-N-methylene-(5,7,4(')-trihydroxy)- isoflavone ameliorates ischemic stroke-induced brain injury in mouse by regulating the oxidative stress and BDNF-Trkb/Akt pathway.

Li Y, Fan F, Liu Q
European journal of pharmacology 2024 Jul 5;974:176512

---

Regulatory role of oxidative stress in retrorsine - Induced apoptosis and autophagy in primary rat hepatocytes.

Zhu Y, Zhang S, Shao Y, Tang L, Zhang C, Tang S, Lu H
Ecotoxicology and environmental safety 2024 Jul 1;279:116515

---

FAXDC2 inhibits the proliferation and invasion of human liver cancer HepG2 cells.

Peng Z, Xu S, Zhang Q, Yang X, Yuan W, Wang Y, Li Y, Zhu P, Wu X, Jiang Z, Li F, Fan X
Experimental and therapeutic medicine 2024 Jan;27(1):27

---

Borneol-Modified Schisandrin B Micelles Cross the Blood-Brain Barrier To Treat Alzheimer's Disease in Aged Mice.

Li FR, Yu Y, Du YM, Kong L, Liu Y, Wang JH, Chen MH, Liu M, Zhang ZX, Li XT, Ju RJ
ACS chemical neuroscience 2024 Feb 7;15(3):593-607

---

Declined circular RNA mitofusin 2 constrains the deterioration of Wilms tumor via modulating microRNA-372-3p/transforming growth factor-β receptor type 2 axis.

Xiao M, Pu X, Tong W, Xiao X
Cellular and molecular biology (Noisy-le-Grand, France) 2024 Feb 29;70(2):143-149

---

Adiponectin attenuates H2O2-induced apoptosis in chicken skeletal myoblasts through the lysosomal-mitochondrial axis.

Wang H, Li C, Zhu L, Liu Z, Li N, Zheng Z, Liang S, Yan J
In vitro cellular & developmental biology. Animal 2024 Aug;60(7):805-814

---

Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production.

Zhang Y, Gao X, Cao M, Xu H, Liu H, Zhao Q, Zhou EM, Chen Y, Liu B
Poultry science 2024 Apr;103(4):103501

---

Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study.

Zheng H, Liang G, Guan C, Liu L, Dong J, Zhao J, Tang M, Kong L
Nanomaterials (Basel, Switzerland) 2024 Apr 17;14(8)

---

Comparison of acute kidney injury following brain death between male and female rats.

Armstrong-Jr R, Ricardo-da-Silva FY, Vidal-Dos-Santos M, da Anunciação LF, Ottens PJ, Correia CJ, Moreira LFP, Leuvenink HGD, Breithaupt-Faloppa AC
Clinics (Sao Paulo, Brazil) 2023;78:100222

---

Effects of Cooling Interventions with Different Target Temperatures on Heat Stroke Rats.

Wu C, Wang P, Wang B, Nijiati M, Hou M
Journal of inflammation research 2023;16:2345-2355

---

Co-exposure to polystyrene microplastics and microcystin-LR aggravated male reproductive toxicity in mice.

Liu H, Jin H, Pan C, Chen Y, Li D, Ding J, Han X
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023 Nov;181:114104

---

Deciphering anti-benign prostatic hyperplasia potential of liangwanoside II based on metabolite profile characterization combined with targeted network pharmacology.

Fan L, Peng Y, Sun C, Ma P, Peng C, Sun A, Li X
Journal of ethnopharmacology 2023 Nov 15;316:116725

---

The combined analgesic, sedative, and anti-gastric cancer mechanisms of Tinospora sagittata var. yunnanensis (S. Y. Hu) H. S. Lo based on integrated ethnopharmacological data.

Wang QQ, Sun QR, Ji XY, Tang Y, Zhang K, Wang XQ, Li HR, Huang XZ, Zhang B
Journal of ethnopharmacology 2023 Mar 1;303:115990

---

An experimental rat model of electric shock injury with isolated electric shock and water conduction: the histopathological changes on the skin and internal organs and the effect on biochemical parameters.

Dündar AS, Oruç M, Celbiş O, Şamdancı ET, Akatlı AN, Okumuş H, Taşkapan Ç, Özhan O, Parlakpınar H
International journal of legal medicine 2023 Jan;137(1):215-226

---

Formononetin improves cardiac function and depressive behaviours in myocardial infarction with depression by targeting GSK-3β to regulate macrophage/microglial polarization.

Yang Y, Huang T, Zhang H, Li X, Shi S, Tian X, Huang Z, Zhang R, Liu Z, Cheng Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 Jan;109:154602

---

Connexin 43-modified bone marrow stromal cells reverse the imatinib resistance of K562 cells via Ca 2+ -dependent gap junction intercellular communication.

Li X, Xiao Y, Wang X, Huang R, Wang R, Deng Y, Rao J, Gao Q, Yang S, Zhang X
Chinese medical journal 2023 Jan 20;136(2):194-206

---

Structural identification of an polysaccharide isolated from Epimedium brevicornum and its beneficial effect on promoting osteogenesis in osteoblasts induced by high glucose.

Lei SS, Li B, Huang XW, Wang XP, Xiong S, Duan R, Li LZ
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Dec 31;169:115893

---

miR-466 Contributes to the Enhanced Antitumor Effect of Bortezomib on Non-Small-Cell Lung Cancer by Inhibiting CCND1.

Wang WH, Zhan JM, Tang YL, Zhou N, Liu WY, Jiang DW
Chemotherapy 2022;67(2):110-122

---

Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challenge.

Yang C, Yuan F, Shao W, Yao L, Jin S, Han F
Genetics and molecular biology 2022;45(4):e20220119

---

lncRNA HOTTIP Recruits EZH2 to Inhibit PTEN Expression and Participates in IM Resistance in Chronic Myeloid Leukemia.

Liu J, Yang L, Liu X, Liu L, Liu M, Feng X, Luo J
Stem cells international 2022;2022:9993393

---

Soluble Epoxide Hydrolase Inhibition Protected against Diabetic Cardiomyopathy through Inducing Autophagy and Reducing Apoptosis Relying on Nrf2 Upregulation and Transcription Activation.

Fang Q, Liu X, Ding J, Zhang Z, Chen G, Du T, Wang Y, Xu R
Oxidative medicine and cellular longevity 2022;2022:3773415

---

Aerobic Exercise Regulates Apoptosis through the PI3K/Akt/GSK-3β Signaling Pathway to Improve Cognitive Impairment in Alzheimer's Disease Mice.

Peng Y, Chi R, Liu G, Tian W, Zhang J, Zhang R
Neural plasticity 2022;2022:1500710

---

TGF-β1 Promotes Autophagy and Inhibits Apoptosis in Breast Cancer by Targeting TP63.

Wang Y, Lu H, Wang Z, Li Y, Chen X
Frontiers in oncology 2022;12:865067

---

Scutellarin Protects Against Mitochondrial Reactive Oxygen Species-Dependent NLRP3 Inflammasome Activation to Attenuate Intervertebral Disc Degeneration.

Wang Z, Zhang P, Zhao Y, Yu F, Wang S, Liu K, Cheng X, Shi J, He Q, Xia Y, Cheng L
Frontiers in bioengineering and biotechnology 2022;10:883118

---

Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways.

Zhang Q, Qian D, Tang DD, Liu J, Wang LY, Chen W, Wu CJ, Peng W
Journal of agricultural and food chemistry 2022 Sep 7;70(35):10782-10793

---

Trilobatin, an Active Dihydrochalcone from Lithocarpus polystachyus, Prevents Cisplatin-Induced Nephrotoxicity via Mitogen-Activated Protein Kinase Pathway-Mediated Apoptosis in Mice.

Duan YY, Mi XJ, Su WY, Tang S, Jiang S, Wang Z, Zhao LC, Li W
ACS omega 2022 Oct 25;7(42):37401-37409

---

Low-dose 5-aza-2'-deoxycytidine protects against early renal injury by increasing klotho expression.

Zhao Y, Zeng X, Xu X, Wang W, Xu L, Wu Y, Li H
Epigenomics 2022 Nov;14(22):1411-1425

---

Cryptotanshinone ameliorates MPP(+)-induced oxidative stress and apoptosis of SH-SY5Y neuroblastoma cells: the role of STAT3 in Parkinson's disease.

Wang Q, Liu Y
Metabolic brain disease 2022 Jun;37(5):1477-1485

---

MiR-153 downregulation alleviates PTSD-like behaviors and reduces cell apoptosis by upregulating the Sigma-1 receptor in the hippocampus of rats exposed to single-prolonged stress.

Chen YL, Tong L, Chen Y, Fu CH, Peng JB, Ji LL
Experimental neurology 2022 Jun;352:114034

---

TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.

Wang Y, Chen Y, Li C, Xiao Z, Yuan H, Zhang Y, Pang D, Tang X, Li M, Ouyang H
Biology 2022 Jan 14;11(1)

---

Integrated chemical profiling, network pharmacology and pharmacological evaluation to explore the potential mechanism of Xinbao pill against myocardial ischaemia-reperfusion injury.

Yang Y, Chen T, Liu J, Chen S, Cai R, Wu L, Hu J, Lin Q, Qi X, Liu Z, Cheng Y
Pharmaceutical biology 2022 Dec;60(1):255-273

---

Thiazolidinedione-Based Structure Modification of Celastrol Provides Thiazolidinedione-Conjugated Derivatives as Potent Agents against Non-Small-Cell Lung Cancer Cells through a Mitochondria-Mediated Apoptotic Pathway.

Fu X, Mao Q, Zhang B, Lv J, Ping K, Zhang P, Lin F, Zhao J, Feng Y, Yang J, Wang H, Zhang L, Mou Y, Wang S
Journal of natural products 2022 Apr 22;85(4):1147-1156

---

The Inhibition of Gastric Cancer Cells' Progression by 23,24-Dihydrocucurbitacin E through Disruption of the Ras/Raf/ERK/MMP9 Signaling Pathway.

Liu H, Wang H, Dong A, Huo X, Wang H, Wang J, Si J
Molecules (Basel, Switzerland) 2022 Apr 22;27(9)

---

Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways.

Cui H, Liu X, Zhang J, Zhang K, Yao D, Dong S, Feng S, Yang L, Li Y, Wang H, Huang J, Wang J
Evidence-based complementary and alternative medicine : eCAM 2021;2021:6103158

---

The novel GLP-1/GIP analogue DA5-CH reduces tau phosphorylation and normalizes theta rhythm in the icv. STZ rat model of AD.

Li C, Liu W, Li X, Zhang Z, Qi H, Liu S, Yan N, Xing Y, Hölscher C, Wang Z
Brain and behavior 2020 Mar;10(3):e01505

---

Crotonaldehyde-induced alterations in testicular enzyme function and hormone levels, and apoptosis in the testes of male Wistar rats are associated with oxidative damage.

Zhang B, Wei P, Men J, Zhang S, Shao H, Zhang Z
Toxicology mechanisms and methods 2020 Jan;30(1):19-32

---

Supplementation of Saponins from Leaves of Panax quinquefolius Mitigates Cisplatin-Evoked Cardiotoxicity via Inhibiting Oxidative Stress-Associated Inflammation and Apoptosis in Mice.

Xing JJ, Hou JG, Liu Y, Zhang RB, Jiang S, Ren S, Wang YP, Shen Q, Li W, Li XD, Wang Z
Antioxidants (Basel, Switzerland) 2019 Sep 1;8(9)

---

Luteolin improves heart preservation through inhibiting hypoxia-dependent L-type calcium channels in cardiomyocytes.

Yan Q, Li Y, Yan J, Zhao Y, Liu Y, Liu S
Experimental and therapeutic medicine 2019 Mar;17(3):2161-2171

---

The antitumor effect of folic acid conjugated-Auricularia auricular polysaccharide-cisplatin complex on cervical carcinoma cells in nude mice.

Qiu J, Zhang H, Wang Z, Liu D, Liu S, Han W, Regenstein JM, Geng L
International journal of biological macromolecules 2018 Feb;107(Pt B):2180-2189

---

Protective Effects of Sodium (±)-5-Bromo-2-(α-Hydroxypentyl) Benzoate in a Rodent Model of Global Cerebral Ischemia.

Gao Y, Li M, Wang Y, Li Z, Fan C, Wang Z, Cao X, Chang J, Qiao H
Frontiers in pharmacology 2017;8:691

---

Nephroprotective Effects of Saponins from Leaves of Panax quinquefolius against Cisplatin-Induced Acute Kidney Injury.

Ma ZN, Li YZ, Li W, Yan XT, Yang G, Zhang J, Zhao LC, Yang LM
International journal of molecular sciences 2017 Jul 13;18(7)

---

Antiproliferative activity of di-2-pyridylhydrazone dithiocarbamate acetate partly involved in p53 mediated apoptosis and autophagy.

Wang T, Liu Y, Fu Y, Huang T, Yang Y, Li S, Li C
International journal of oncology 2017 Dec;51(6):1909-1919

---

Ouabain targets the Na(+)/K(+)-ATPase α(3) isoform to inhibit cancer cell proliferation and induce apoptosis.

Xiao Y, Meng C, Lin J, Huang C, Zhang X, Long Y, Huang Y, Lin Y
Oncology letters 2017 Dec;14(6):6678-6684

---

Baicalin Attenuates Alcoholic Liver Injury through Modulation of Hepatic Oxidative Stress, Inflammation and Sonic Hedgehog Pathway in Rats.

Wang H, Zhang Y, Bai R, Wang M, Du S
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2016;39(3):1129-40

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of colon, using Bax Antibody.

Additional image