Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 625101 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#625101, RRID:AB_315997
- Product name
- Purified anti-Bax
- Antibody type
- Polyclonal
- Antigen
- Peptide-KLH, N-terminal
- Description
- Bax is a 21 kD pro-apoptotic protien known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, the ovary, and in the lymph node. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. The Poly6251 antibody has been reported to be useful for Western blotting, immunoprecipitation, and immunohistochemistry of human, mouse, and rat Bax protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 50 µl
- Storage
- The antibody solution should be stored undiluted at 4°C. DO NOT FREEZE.
Submitted references Mitochondrial biogenesis fails in secondary biliary cirrhosis in rats leading to mitochondrial DNA depletion and deletions.
Arduini A, Serviddio G, Escobar J, Tormos AM, Bellanti F, ViƱa J, Monsalve M, Sastre J
American journal of physiology. Gastrointestinal and liver physiology 2011 Jul;301(1):G119-27
American journal of physiology. Gastrointestinal and liver physiology 2011 Jul;301(1):G119-27
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Bax polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
- Conjugate
- Unconjugated