633601

antibody from BioLegend

Targeting: BAX BCL2L4

Western blot (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

633601

Provider

BioLegend

Proper citation

BioLegend Cat#633601, RRID:AB_961011

Product name

Purified anti-Bax

Antibody type

Monoclonal

Antigen

Amino acid: 3-16 of human Bax protein

Description

Bax is a 21 kD pro-apoptotic protein known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, the ovary, and in the lymph node. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. Clone 2D2 has been shown to be useful for western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry of the human Bax protein.This antibody does not cross-react with Bcl-2 or Bcl-XL proteins.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

Upon receipt, store undiluted at 4°C.