Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- MA5-16322 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bax Monoclonal Antibody (SP47)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is normal breast tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP47
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references NF-κB RNAi decreases the Bax/Bcl-2 ratio and inhibits TNF-α-induced apoptosis in human alveolar epithelial cells.
Characterization of cell clones stably transfected with short form caspase-9: apoptotic resistance and Bcl-XL expression.
Li L, Wu W, Huang W, Hu G, Yuan W, Li W
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2013 Apr;62(4):387-97
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2013 Apr;62(4):387-97
Characterization of cell clones stably transfected with short form caspase-9: apoptotic resistance and Bcl-XL expression.
Yi X, Wang J, Seol DW, Dong Z
Molecular and cellular biochemistry 2006 Jan;282(1-2):1-12
Molecular and cellular biochemistry 2006 Jan;282(1-2):1-12
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of BAX using anti-BAX Monoclonal Antibody (Product # MA5-16322) in Normal Breast Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:50.