MA5-16322

antibody from Invitrogen Antibodies

Targeting: BAX BCL2L4

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-16322

Provider

Invitrogen Antibodies

Product name

Bax Monoclonal Antibody (SP47)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is normal breast tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

SP47

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

NF-κB RNAi decreases the Bax/Bcl-2 ratio and inhibits TNF-α-induced apoptosis in human alveolar epithelial cells.

Li L, Wu W, Huang W, Hu G, Yuan W, Li W
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2013 Apr;62(4):387-97

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Characterization of cell clones stably transfected with short form caspase-9: apoptotic resistance and Bcl-XL expression.

Yi X, Wang J, Seol DW, Dong Z
Molecular and cellular biochemistry 2006 Jan;282(1-2):1-12

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BAX using anti-BAX Monoclonal Antibody (Product # MA5-16322) in Normal Breast Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:50.