Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [1]
- Other assay [5]
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- Product number
- PA5-11378 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bax Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1.6 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Beneficial effect of trimetazidine on folic acid-induced acute kidney injury in mice: Role of HIF-1α/HO-1.
In vivo analysis of onset and progression of retinal degeneration in the Nr2e3(rd7/rd7) mouse model of enhanced S-cone sensitivity syndrome.
Nicorandil mitigates folic acid-induced nephrotoxicity in mice: Role of iNOS and eNOS.
Anti-apoptotic effect of vinpocetine on cisplatin-induced hepatotoxicity in mice: The role of Annexin-V, Caspase-3, and Bax.
TMEM119 silencing inhibits cell viability and causes the apoptosis of gastric cancer SGC-7901 cells.
c-Rel deficiency increases caspase-4 expression and leads to ER stress and necrosis in EBV-transformed cells.
Abdelrahman RS, Abdelsalam RA, Zaghloul MS
Journal of biochemical and molecular toxicology 2022 May;36(5):e23011
Journal of biochemical and molecular toxicology 2022 May;36(5):e23011
In vivo analysis of onset and progression of retinal degeneration in the Nr2e3(rd7/rd7) mouse model of enhanced S-cone sensitivity syndrome.
Venturini G, Kokona D, Steiner BL, Bulla EG, Jovanovic J, Zinkernagel MS, Escher P
Scientific reports 2021 Sep 24;11(1):19032
Scientific reports 2021 Sep 24;11(1):19032
Nicorandil mitigates folic acid-induced nephrotoxicity in mice: Role of iNOS and eNOS.
Ezzat DM, Soliman AM, El-Kashef DH
Journal of biochemical and molecular toxicology 2021 Apr;35(4):e22692
Journal of biochemical and molecular toxicology 2021 Apr;35(4):e22692
Anti-apoptotic effect of vinpocetine on cisplatin-induced hepatotoxicity in mice: The role of Annexin-V, Caspase-3, and Bax.
Habib SA, Abdelrahman RS, Abdel Rahim M, Suddek GM
Journal of biochemical and molecular toxicology 2020 Oct;34(10):e22555
Journal of biochemical and molecular toxicology 2020 Oct;34(10):e22555
TMEM119 silencing inhibits cell viability and causes the apoptosis of gastric cancer SGC-7901 cells.
Zheng P, Wang W, Ji M, Zhu Q, Feng Y, Zhou F, He Q
Oncology letters 2018 Jun;15(6):8281-8286
Oncology letters 2018 Jun;15(6):8281-8286
c-Rel deficiency increases caspase-4 expression and leads to ER stress and necrosis in EBV-transformed cells.
Valentín-Acevedo A, Sinquett FL, Covey LR
PloS one 2011;6(10):e25467
PloS one 2011;6(10):e25467
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a Bax polyclonal antibody (Product # PA5-11378) in HL-60 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Bax (arrow) using a Bax polyclonal antibody (Product # PA5-11378) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bax gene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Bax (arrow) using a Bax polyclonal antibody (Product # PA5-11378) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bax gene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Flow cytometry analysis of HepG2 cells using a Bax polyclonal antibody (Product # PA5-11378) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 3. TMEM119 silencing induced SGC-7901 cell apoptosis. After transfection of SGC-7901 cells with TMEM119-siRNA (siTMEM119), cell apoptosis was measured by (A and B) flow cytometry assay and the expression of Bcl-2, Bax as well as caspase-3 was measured by (C and D) western blot analysis. **P
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- 4 Figure Effect of Vin on apoptotic parameters. (A) Flow cytometry of Annexin-V; (B) flow cytometry of caspase-3; (C) representative photomicrographs of immunohistochemical detection of BAX in the liver (x400; scale bar = 50 um) (left panel) and bar graphs showing the percentage of BAX-immunopositive cells (right panel), Bax immune-stained liver tissue. The data shown are mean +- SEM ( n = 6). * $# P < .05 compared with control group, Cis group, and Vin10 + Cis group, respectively. Statistical analysis was performed using one-way analysis of variance test followed by Tukey's multiple comparison test. Cis, cisplatin; SEM, standard error of the mean; Vin, vinpocetine
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 5 Figure Effect of nicorandil (50 and 100 mg/kg) on Bax expression. A and B, Control group: showed negative staining. C and D, FA group: showed marked increase in positive immunoreactivity of renal tubules against Bax (black arrows). E and F, Nico 50 + FA group: revealed moderate expression of Bax. G and H, Nico 100 + FA group: exhibited mild expression of Bax. IHC counterstained with Mayer's hematoxylin, X:100 bar 100 um (A, C, E, and G) and X: 400 bar 50 um (B, D, F, and H). I, Statistical analysis was carried out using Kruskal-Wallis test followed by Dunn post hoc test $ p < .05 versus control; # p < .05 versus FA
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- Invitrogen Antibodies (provider)
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- Figure 6 Cell death mechanisms in C57BL/6J Nr2e3 rd7/rd7 retinas. Immunohistochemical analysis of cell death markers on 5 muM-paraffin sections of 1-year-old (1y) C57BL/6J Nr2e3 rd7/rd7 (rd7) ( A , C , E , G , I , K , M ) and wild-type C57BL/6J (BL6) ( B , D , F , H , J , L , N ) retinas. Sections were probed with antibodies raised against Bax ( A , B ), cleaved Caspase-3 ( C , D ), cleaved PARP-1 ( E , F ), MLKL ( G , H ), PAR ( I , J ), Calpain-2 ( K , L ) and Survivin ( M , N ). Bax, cleaved Caspase-3, MLKL and Survivin were detected with a secondary antibody conjugated to Alexa Fluor 594 (red), cleaved PARP-1 and Calpain-2 with a secondary antibody conjugated to Cy5 (red) and PAR with a secondary antibody conjugated to FITC (green). Nuclei were stained with DAPI (blue), namely the outer nuclear layer (ONL) and the inner nuclear layer (INL). Scale bar: 50 um. ( O ) Qualitative Western blot analysis on six pooled retinas of 21-day (P21) and 1-year-old (1y) C57BL/6J (BL6) and C57BL/6J Nr2e3 rd7/rd7 (rd7) mice. Expression of the 16-kDa survivin and 49-kDa alpha-tubulin proteins were assessed.
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- 3 Figure Effect of trimetazidine (20 mg/kg) on kidney apoptotic markers induced by folic acid in mice. Upper panel: Representative photomicrographs of immunohistochemical detection of proapoptotic Bax (left panel); antiapoptotic Bcl2 (middle panel); caspase-3 (right panel) (x100, bar = 100 um). Lower panel: bar graphs showing area of proapoptotic Bax (left panel); antiapoptotic Bcl2 (middle panel); caspase-3 (right panel) positive staining statistical significances of data (mean +- SEM, n = 4) were denoted as *, $ ( p < 0.05) from control groups and folic acid groups, respectively, using one-way ANOVA followed by Tukey-Kramer multiple comparisons test. ANOVA, analysis of variance; CON, control; FA, folic acid TMZ, trimetazidine