LS-C290525
antibody from LSBio
Targeting: SMARCA4
BAF190, BRG1, FLJ39786, hSNF2b, SNF2, SNF2-BETA, SNF2L4, SNF2LB, SWI2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- LS-C290525 - Provider product page
- Provider
- LSBio
- Product name
- SMARCA4 / BRG1 Antibody (aa50-100) LS-C290525
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Storage
- Store at 2-8°C for up to one year.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human and Mouse BRG1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified goat anti-BRG1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human PAPD1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg), HEK293T (50µg), and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-PAPD1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human BRG1 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-BRG1 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Mouse BRG1 by Immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BRG1 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.