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LearnGTX633391
antibody from GeneTex
Targeting: SMARCA4
BAF190, BRG1, FLJ39786, hSNF2b, SNF2, SNF2-BETA, SNF2L4, SNF2LB, SWI2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- GTX633391 - Provider product page
- Provider
- GeneTex
- Product name
- BRG1 antibody [GT2712]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and BRG1 knockout (KO) 293T cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BRG1 antibody [GT2712] detects BRG1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BRG1 protein stained by BRG1 antibody [GT2712] (GTX633391) diluted at 1:100.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BRG1 antibody [GT2712] detects BRG1 protein by immunohistochemical analysis.Sample: Frozen-sectioned rat E13.5 brain.Green: BRG1 stained by BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.Red: SOX2, stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
