10556-1-AP

antibody from Proteintech Group

Targeting: NCL C23, Nsr1

Provider product page for 10556-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [35]
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Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 10556-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10556-1-AP, RRID:AB_2082423
Product name: NCL antibody
Antibody type: Polyclonal
Description: NCL antibody (Cat. #10556-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NCL protein structure - 10556-1-AP shown in red.

Submitted references Plasma exosomes proteome profiling discovers protein markers associated with the therapeutic effect of Chaihu-Longgu-Muli decoction on temporal lobe epilepsy.

Yang P, Huang Y, Zhu Y, Wang Q, Guo Y, Li L
Journal of ethnopharmacology 2024 Jan 10;318(Pt A):116928

DHX9 maintains epithelial homeostasis by restraining R-loop-mediated genomic instability in intestinal stem cells.

Ren X, Liu Q, Zhou P, Zhou T, Wang D, Mei Q, Flavell RA, Liu Z, Li M, Pan W, Zhu S
Nature communications 2024 Apr 9;15(1):3080

HMGB1 prefers to interact with structural RNAs and regulates rRNA methylation modification and translation in HeLa cells.

Liao M, Cao J, Chen W, Wang M, Jin Z, Ye J, Ren Y, Wei Y, Xue Y, Chen D, Zhang Y, Chen S
BMC genomics 2024 Apr 5;25(1):345

Nucleophilic covalent ligand discovery for the cysteine redoxome.

Fu L, Jung Y, Tian C, Ferreira RB, Cheng R, He F, Yang J, Carroll KS
Nature chemical biology 2023 Nov;19(11):1309-1319

PICH Activates Cyclin A1 Transcription to Drive S-Phase Progression and Chemoresistance in Gastric Cancer.

Ye H, Shi W, Yang J, Wang L, Jiang X, Zhao H, Qin L, Qin J, Li L, Cai W, Guan J, Yang H, Zhou H, Yu Z, Sun H, Jiao Z
Cancer research 2023 Nov 15;83(22):3767-3782

Nucleolus assembly impairment leads to two-cell transcriptional repression via NPM1-mediated PRC2 recruitment.

Li D, Cao R, Li Q, Yang Y, Tang A, Zhang J, Liu Q
Nature structural & molecular biology 2023 Jul;30(7):914-925

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing.

Chen QW, Cai QQ, Yang Y, Dong S, Liu YY, Chen ZY, Kang CL, Qi B, Dong YW, Wu W, Zhuang LP, Shen YH, Meng ZQ, Wu XZ
Clinical and translational medicine 2023 Jan;13(1):e1129

Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth.

Gu C, Zhang W, Yang E, Gu C, Zhang Z, Ke J, Wang X, Wu S, Li S, Wu F
Oncogenesis 2022 Sep 15;11(1):55

A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin β1 Interactomes.

Song DA, Alber S, Doron-Mandel E, Schmid V, Albus CA, Leitner O, Hamawi H, Oses-Prieto JA, Dezorella N, Burlingame AL, Fainzilber M, Rishal I
Molecular & cellular proteomics : MCP 2022 Nov;21(11):100418

Human histone pre-mRNA assembles histone or canonical mRNA-processing complexes by overlapping 3'-end sequence elements.

Ielasi FS, Ternifi S, Fontaine E, Iuso D, Couté Y, Palencia A
Nucleic acids research 2022 Nov 28;50(21):12425-12443

Nucleolus localization of SpyCas9 affects its stability and interferes with host protein translation in mammalian cells.

Tan R, Du W, Liu Y, Cong X, Bai M, Jiang C, Li Z, Tan M, Ma DK, Huang Q, Jiang W, Dang Y
Genes & diseases 2022 May;9(3):731-740

Antioxidant and Immunomodulatory Properties of Partially purified Exopolysaccharide from Lactobacillus Casei Isolated from Chinese Northeast Sauerkraut.

Xu X, Qiao Y, Peng Q, Shi B, Dia VP
Immunological investigations 2022 May;51(4):748-765

Downregulation of NCL attenuates tumor formation and growth in HeLa cells by targeting the PI3K/AKT pathway.

Ying J, Pan R, Tang Z, Zhu J, Ren P, Lou Y, Zhang E, Huang D, Hu P, Li D, Bao Q, Li P
Cancer medicine 2022 Mar;11(6):1454-1464

Fast detection, a precise and sensitive diagnostic agent for breast cancer.

Wu Q, Yuan C, Liu N, Shu J, Wang J, Qian J, Zeng L, Zhang H, Wang X, Mei W
Journal of experimental & clinical cancer research : CR 2022 Jun 13;41(1):201

Self-Assembled DNA Nanostructure as a Carrier for Targeted siRNA Delivery in Glioma Cells.

Zhou Y, Yang Q, Wang F, Zhou Z, Xu J, Cheng S, Cheng Y
International journal of nanomedicine 2021;16:1805-1817

The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.

Doron-Mandel E, Koppel I, Abraham O, Rishal I, Smith TP, Buchanan CN, Sahoo PK, Kadlec J, Oses-Prieto JA, Kawaguchi R, Alber S, Zahavi EE, Di Matteo P, Di Pizio A, Song DA, Okladnikov N, Gordon D, Ben-Dor S, Haffner-Krausz R, Coppola G, Burlingame AL, Jungwirth P, Twiss JL, Fainzilber M
The EMBO journal 2021 Oct 18;40(20):e107158

Overexpression of ARHGAP30 suppresses growth of cervical cancer cells by downregulating ribosome biogenesis.

Wu A, Lin L, Li X, Xu Q, Xu W, Zhu X, Teng Y, Yang XM, Ai Z
Cancer science 2021 Nov;112(11):4515-4525

Nuclear m(6)A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos.

Chen C, Liu W, Guo J, Liu Y, Liu X, Liu J, Dou X, Le R, Huang Y, Li C, Yang L, Kou X, Zhao Y, Wu Y, Chen J, Wang H, Shen B, Gao Y, Gao S
Protein & cell 2021 Jun;12(6):455-474

Inhibition of Specificity Protein 1 Is Involved in Phloretin-Induced Suppression of Prostate Cancer.

Kang D, Zuo W, Wu Q, Zhu Q, Liu P
BioMed research international 2020;2020:1358674

ERp57‑small interfering RNA silencing can enhance the sensitivity of drug‑resistant human ovarian cancer cells to paclitaxel.

Li S, Zhao X, Chang S, Li Y, Guo M, Guan Y
International journal of oncology 2019 Jan;54(1):249-260

The nuclear matrix protein HNRNPU maintains 3D genome architecture globally in mouse hepatocytes.

Fan H, Lv P, Huo X, Wu J, Wang Q, Cheng L, Liu Y, Tang QQ, Zhang L, Zhang F, Zheng X, Wu H, Wen B
Genome research 2018 Feb;28(2):192-202

C9ORF72 hexanucleotide repeat exerts toxicity in a stable, inducible motor neuronal cell model, which is rescued by partial depletion of Pten.

Stopford MJ, Higginbottom A, Hautbergue GM, Cooper-Knock J, Mulcahy PJ, De Vos KJ, Renton AE, Pliner H, Calvo A, Chio A, Traynor BJ, Azzouz M, Heath PR, ITALSGEN Consortium, NeuroX Consortium, Kirby J, Shaw PJ
Human molecular genetics 2017 Mar 15;26(6):1133-1145

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

Bennion Callister J, Ryan S, Sim J, Rollinson S, Pickering-Brown SM
Human molecular genetics 2016 Dec 1;25(23):5069-5082

Upregulation of miR-497 induces hepatic insulin resistance in E3 rats with HFD-MetS by targeting insulin receptor.

Wang X, Wang M, Li H, Lan X, Liu L, Li J, Li Y, Li J, Yi J, Du X, Yan J, Han Y, Zhang F, Liu M, Lu S, Li D
Molecular and cellular endocrinology 2015 Nov 15;416:57-69

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Tao Z, Wang H, Xia Q, Li K, Li K, Jiang X, Xu G, Wang G, Ying Z
Human molecular genetics 2015 May 1;24(9):2426-41

Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.

Cooper-Knock J, Higginbottom A, Stopford MJ, Highley JR, Ince PG, Wharton SB, Pickering-Brown S, Kirby J, Hautbergue GM, Shaw PJ
Acta neuropathologica 2015 Jul;130(1):63-75

Phosphorylation and changes in the distribution of nucleolin promote tumor metastasis via the PI3K/Akt pathway in colorectal carcinoma.

Wu DM, Zhang P, Liu RY, Sang YX, Zhou C, Xu GC, Yang JL, Tong AP, Wang CT
FEBS letters 2014 May 21;588(10):1921-9

C9orf72 nucleotide repeat structures initiate molecular cascades of disease.

Haeusler AR, Donnelly CJ, Periz G, Simko EA, Shaw PG, Kim MS, Maragakis NJ, Troncoso JC, Pandey A, Sattler R, Rothstein JD, Wang J
Nature 2014 Mar 13;507(7491):195-200

Antisense proline-arginine RAN dipeptides linked to C9ORF72-ALS/FTD form toxic nuclear aggregates that initiate in vitro and in vivo neuronal death.

Wen X, Tan W, Westergard T, Krishnamurthy K, Markandaiah SS, Shi Y, Lin S, Shneider NA, Monaghan J, Pandey UB, Pasinelli P, Ichida JK, Trotti D
Neuron 2014 Dec 17;84(6):1213-25

14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage.

Tang S, Bao H, Zhang Y, Yao J, Yang P, Chen X
PloS one 2013;8(3):e55268

14-3-3ε boosts bleomycin-induced DNA damage response by inhibiting the drug-resistant activity of MVP.

Tang S, Bai C, Yang P, Chen X
Journal of proteome research 2013 Jun 7;12(6):2511-24

Nucleocapsid protein VP15 of White spot syndrome virus colocalizes with the nucleolar proteins nucleolin and fibrillarin.

Xing Y, Shi Z
Canadian journal of microbiology 2011 Sep;57(9):759-64

Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma.

Yang X, Zou P, Yao J, Yun D, Bao H, Du R, Long J, Chen X
Journal of proteome research 2010 Mar 5;9(3):1402-15

Subcellular quantitative proteomics reveals multiple pathway cross-talk that coordinates specific signaling and transcriptional regulation for the early host response to LPS.

Du R, Long J, Yao J, Dong Y, Yang X, Tang S, Zuo S, He Y, Chen X
Journal of proteome research 2010 Apr 5;9(4):1805-21

Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.

Bao H, Song P, Liu Q, Liu Y, Yun D, Saiyin H, Du R, Zhang Y, Fan H, Yang P, Chen X
Proteomics. Clinical applications 2009 Jun;3(6):705-19

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10556-1-AP(NCL antibody) at dilution of 1:2000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The NCL antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NCL. This antibody recognizes human, mouse antigen. The NCL antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 10556-1-AP.pdf

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