10556-1-AP

antibody from Proteintech Group

Targeting: NCL C23, Nsr1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10556-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10556-1-AP, RRID:AB_2082423

Product name

NCL antibody

Antibody type

Polyclonal

Description

NCL antibody (Cat. #10556-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Nucleolar ribosomal RNA synthesis continues in differentiating lens fiber cells until abrupt nuclear degradation required for ocular lens transparency.

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Integrative analysis and experimental validation identify the role of CD44 and Nucleolin in regulating gliogenesis following spinal cord injury.

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Subcellular proteomics reveals the crosstalk between nucleocytoplasmic trafficking and the innate immune response to Senecavirus A infection.

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Nucleolin malonylation as a nuclear-cytosol signal exchange mechanism to drive cell proliferation in Hepatocarcinoma by enhancing AKT translation.

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Plasma exosomes proteome profiling discovers protein markers associated with the therapeutic effect of Chaihu-Longgu-Muli decoction on temporal lobe epilepsy.

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Long non-coding RNA Snhg15 promotes preosteoblast proliferation by interacting with and stabilizing nucleolin.

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Liao M, Cao J, Chen W, Wang M, Jin Z, Ye J, Ren Y, Wei Y, Xue Y, Chen D, Zhang Y, Chen S
BMC genomics 2024 Apr 5;25(1):345

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Immunological investigations 2022 May;51(4):748-765

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Downregulation of NCL attenuates tumor formation and growth in HeLa cells by targeting the PI3K/AKT pathway.

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Nucleolin Promotes Cisplatin Resistance in Cervical Cancer by the YB1-MDR1 Pathway.

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Self-Assembled DNA Nanostructure as a Carrier for Targeted siRNA Delivery in Glioma Cells.

Zhou Y, Yang Q, Wang F, Zhou Z, Xu J, Cheng S, Cheng Y
International journal of nanomedicine 2021;16:1805-1817

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The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.

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Inhibition of Specificity Protein 1 Is Involved in Phloretin-Induced Suppression of Prostate Cancer.

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ERp57‑small interfering RNA silencing can enhance the sensitivity of drug‑resistant human ovarian cancer cells to paclitaxel.

Li S, Zhao X, Chang S, Li Y, Guo M, Guan Y
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Human molecular genetics 2016 Dec 1;25(23):5069-5082

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Molecular and cellular endocrinology 2015 Nov 15;416:57-69

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Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Tao Z, Wang H, Xia Q, Li K, Li K, Jiang X, Xu G, Wang G, Ying Z
Human molecular genetics 2015 May 1;24(9):2426-41

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Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.

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Acta neuropathologica 2015 Jul;130(1):63-75

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Phosphorylation and changes in the distribution of nucleolin promote tumor metastasis via the PI3K/Akt pathway in colorectal carcinoma.

Wu DM, Zhang P, Liu RY, Sang YX, Zhou C, Xu GC, Yang JL, Tong AP, Wang CT
FEBS letters 2014 May 21;588(10):1921-9

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C9orf72 nucleotide repeat structures initiate molecular cascades of disease.

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Antisense proline-arginine RAN dipeptides linked to C9ORF72-ALS/FTD form toxic nuclear aggregates that initiate in vitro and in vivo neuronal death.

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Tang S, Bai C, Yang P, Chen X
Journal of proteome research 2013 Jun 7;12(6):2511-24

---

Nucleocapsid protein VP15 of White spot syndrome virus colocalizes with the nucleolar proteins nucleolin and fibrillarin.

Xing Y, Shi Z
Canadian journal of microbiology 2011 Sep;57(9):759-64

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Yang X, Zou P, Yao J, Yun D, Bao H, Du R, Long J, Chen X
Journal of proteome research 2010 Mar 5;9(3):1402-15

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Subcellular quantitative proteomics reveals multiple pathway cross-talk that coordinates specific signaling and transcriptional regulation for the early host response to LPS.

Du R, Long J, Yao J, Dong Y, Yang X, Tang S, Zuo S, He Y, Chen X
Journal of proteome research 2010 Apr 5;9(4):1805-21

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Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.

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Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10556-1-AP(NCL antibody) at dilution of 1:2000

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The NCL antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NCL. This antibody recognizes human, mouse antigen. The NCL antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

10556-1-AP.pdf

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