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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324425 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal NCL Antibody (Center E443)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal NCL Antibody (Center E443)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NCL
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.31 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- NCL Antibody (Center E443) (Cat. #TA324425) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of NCL (arrow) using rabbit polyclonal NCL Antibody (Center E443) (Cat. #TA324425). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with NCL Antibody (Center E443)(Cat#TA324425). Hela cells were incubated with NCL primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . NCL immunoreactivity is localized to nucleus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- NCL Antibody (Center E443) (Cat. #TA324425)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCL Antibody (Center E443) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
