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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- TA324557 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal NCL Antibody (Center P291)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal NCL Antibody (Center P291)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NCL
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- NCL Antibody (Center P291) (Cat. #TA324557) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cell stained with NCL Antibody (Center P291)(Cat#TA324557).U251 cells were incubated with NCL primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . NCL immunoreactivity is localized to Nucleus significantly.
- Validation comment
- IF
