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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP52825PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52825PU-N, RRID:AB_11146020
- Product name
- anti Nucleolin (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 435-463 amino acids from the Central region of Human Nucleolin.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of NCL (arrow) using Nucleolin Antibody (Center) Cat.-No AP52825PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Nucleolin Antibody (Center) Cat.-No AP52825PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the NCL antibody detected the NCL protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Nucleolin Antibody (Center) Cat.-No AP52825PU-N with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human breast tissue stained with Nucleolin Antibody (Center) Cat.-No AP52825PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
