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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F41619 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Nucleolin Antibody
- Antibody type
- Polyclonal
- Antigen
- The amino acid sequence used as immunogen for this Nucleolin antibody is found between 425-455 of the human protein.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- The Nucleolin antibody should be aliquoted and and stored frozen at -20°C. Avoid repeat freeze-thaws.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Nucleolin antibody western blot analysis in HepG2 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of Nucleolin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the NCL gene.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of HeLa cell stained with Nucleolin antibody at 1:25. NCL immunoreactivity is localized to the nucleus.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Nucleolin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue.
