Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
- Flow cytometry [3]
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Validation data
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- Product number
- NBP2-47860-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Nucleolin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - Human Bladder carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - Human Uterus stained with Nucleolin Monoclonal Antibody (NCL/902).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - Human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - Human Tonsil stained with Nucleolin Monoclonal Antibody (NCL/902).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - Human Testicular Carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Antibody (NCL/902).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - An intracellular stain was performed on RH-30 cells with Nucleolin Antibody [NCL/902] NBP2-47860F647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - Flow Cytometry of Human Nucleolin Ag on Jurkat cells. Grey: Isotype Control; Green: FITC-labeled; Purple: APC-Labeled; Turquoise: AF700 Labeled Nucleolin Antibody (NCL/902).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Nucleolin Antibody (NCL/902) - Azide and BSA Free [NBP2-47860] - Flow Cytometry of Human Nucleolin Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Antibody (NCL/902).