Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [3]
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- Product number
- NBP2-48319 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Nucleolin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 7 ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) - IHC-Prediluted [NBP2-48319] - Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Monoclonal Antibody (364-5).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) - IHC-Prediluted [NBP2-48319] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) - IHC-Prediluted [NBP2-48319] - Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 conjugate of Nucleolin Monoclonal Antibody (364-5).