M00228-1

antibody from Boster Biological Technology

Targeting: NCL C23, Nsr1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00228-1

Provider

Boster Biological Technology

Product name

Anti-Nucleolin Monoclonal Antibody (4E2)

Antibody type

Monoclonal

Description

Mouse Monoclonal 400 antibody for NCL detection. Tested positive for Flow Cytometry, IF, IHC, WB in Human

Reactivity

Human

Host

Mouse

Antibody clone number

400

Vial size

100µg

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

-20°C

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of NCL using anti-NCL antibody (M00228-1).NCL was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NCL Antibody (M00228-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Additional image