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- Antibody Data
- Antigen structure
- References [2]
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- Western blot [1]
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- Product number
- 609404 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#609404, RRID:AB_2150741
- Product name
- Purified anti-Nucleolin-Phospho (Thr76/Thr84)
- Antibody type
- Monoclonal
- Antigen
- Modified peptide
- Description
- Nucleolin is a ubiquitously expressed 100 kD nuclear and nucleolar protein that contains RNA recognition motifs. Nucleoloin is the major nucleolar protein of eukaryotic cells in growth phase associated with intranucleolar chromatin and pre-ribosomal particles. This protein induces chromatin de-condensation by binding to histone H1 and plays a role in pre-rRNA transcription and ribosome assembly. Nucleolin exhibits self-cleaving, DNA helicase, RNA helicase and DNA-dependent ATPase activities; activity can be upregulated in response to ERK signaling, c-Myc expression, and genotoxic stress (UV radiation). This protein is modified by phosphorylation (Thr76/Thr84). This protein interacts with phosphatase 1δ, hepatitis C virus NS5B, part of B-cell specific transcription factor complex with LR1, and DNA Topoisomerase I. The 10C7 monoclonal antibody recognizes human phosphorylated nucleolin (Thr76/Thr84) and has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgM
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store at 4°C. Do not freeze.
Submitted references Interplay between REST and nucleolin transcription factors: a key mechanism in the overexpression of genes upon increased phosphorylation.
Targeting neural-restrictive silencer factor sensitizes tumor cells to antibody-based cancer immunotherapy in vitro via multiple mechanisms.
Tediose T, Kolev M, Sivasankar B, Brennan P, Morgan BP, Donev R
Nucleic acids research 2010 May;38(9):2799-812
Nucleic acids research 2010 May;38(9):2799-812
Targeting neural-restrictive silencer factor sensitizes tumor cells to antibody-based cancer immunotherapy in vitro via multiple mechanisms.
Kolev MV, Ruseva MM, Morgan BP, Donev RM
Journal of immunology (Baltimore, Md. : 1950) 2010 Jun 1;184(11):6035-42
Journal of immunology (Baltimore, Md. : 1950) 2010 Jun 1;184(11):6035-42
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- Experimental details
- Formalin-fixed paraffin-embedded human testis tissue was stained with 10C7 at 10 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with Hematoxylin (blue). Magnification, 40X.
- Conjugate
- Unconjugated
