V8771

antibody from NSJ Bioreagents

Targeting: NCL C23, Nsr1

Immunocytochemistry

Flow cytometry

Antibody data

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Validation data

Product number: V8771 - Provider product page
Provider: NSJ Bioreagents
Product name: Nucleolin Antibody
Antibody type: Monoclonal
Description: This highly specific Nucleolin antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human samples.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antibody clone number: NCL/7014R
Vial size: 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
Concentration: 0.2 mg/ml, 1 mg/ml
Storage: Store the Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

NCL protein structure - V8771 shown in red.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC staining of FFPE human salivary gland with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC staining of FFPE human kidney tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC staining of FFPE human kidney tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Negative: IHC staining of FFPE mouse stomach tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Negative: IHC staining of FFPE cat liver tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Negative: IHC staining of FFPE horse lung tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Negative: IHC staining of FFPE cow heart tissue with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.