NBP2-19535
antibody from Novus Biologicals
Targeting: NFYB
CBF-A, HAP3, NF-YB
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-19535 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal NFYB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFYB Antibody [NBP2-19535] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFYB Antibody [NBP2-19535] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFYB Antibody [NBP2-19535] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NFYB Antibody [NBP2-19535] - 293T whole cell lysate/extract A : 40 ug whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 ug of NFYB antibody 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) as used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Paraffin-embedded mouse intestine. NFYB antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Paraffin-embedded rat stomach. NFYB antibody diluted at 1:500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP): NFYB Antibody [NBP2-19535] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.ChIP experiment and primer designs are based on Nucleic Acids Res. 2006;34(21):6272-85.