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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX121922 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX121922, RRID:AB_10722365
- Product name
- NFYB antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ?g) A: HeLa nucleus 12% SDS PAGE GTX121922 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa whole cell and nuclear extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFYB(GTX121922) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NFYB antibody detects NFYB protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NFYB protein stained by NFYB antibody (GTX121922) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NFYB antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 £gg whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 £gg of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 £gg of NFYB antibody (GTX121922) 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody (GTX121922) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NFYB antibody detects NFYB protein at nucleus in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. NFYB antibody (GTX121922) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NFYB antibody detects NFYB protein at nucleus in rat stomach by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. NFYB antibody (GTX121922) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 £gg of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.
