H00004801-M01

antibody from Abnova Corporation

Targeting: NFYB CBF-A, HAP3, NF-YB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004801-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004801-M01, RRID:AB_518950

Product name

NFYB monoclonal antibody (M01), clone 6H6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant NFYB.

Antigen sequence

MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDT
EDSMNDHEDTNGSKESFREQDIYLPIANVARIMKN
AIPQTGKIAKDAKECVQECVSEFISFITSEASERC
HQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQK
FREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLP
AGLITTDGQQQNVMVYTTSYQQISGVQQIQFS


Isotype

IgG

Antibody clone number

6H6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NFYB is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol