PA5-30102

antibody from Invitrogen Antibodies

Targeting: NFYB CBF-A, HAP3, NF-YB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30102

Provider

Invitrogen Antibodies

Product name

NFYB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (91%), Pig (99%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NFYB Polyclonal Antibody (Product # PA5-30102). Sample (20 µg ). Lane A: HeLa. 12% SDS PAGE. NFYB Polyclonal Antibody (Product # PA5-30102) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NFYB in paraformaldehyde-fixed HeLa cells using a NFYB polyclonal antibody (Product # PA5-30102) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of NFYB was performed in paraffin-embedded mouse liver tissue using NFYB Polyclonal Antibody (Product # PA5-30102) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using NFYB (Product # PA5-30102) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.