PA5-31913

antibody from Invitrogen Antibodies

Targeting: NFYB CBF-A, HAP3, NF-YB

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-31913 - Provider product page
Provider: Invitrogen Antibodies
Product name: NFYB Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: HeLa, HeLa nuclear extract, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (92%), Pig (99%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.42 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

NFYB protein structure - PA5-31913 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NFYB using 20 µg of HeLa nucleus lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a NFYB polyclonal antibody (Product # PA5-31913) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of NFYB was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using NFYB Polyclonal Antibody (Product # PA5-31913). HeLa whole cell and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB Polyclonal Antibody (Product # PA5-31913) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of NFYB was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of NFYB was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of NFYB was performed by separating 30 µg of HeLa whole cell and nuclear extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using NFYB Polyclonal Antibody (Product # PA5-31913). HeLa whole cell and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB Polyclonal Antibody (Product # PA5-31913) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.