PA5-31913

antibody from Invitrogen Antibodies

Targeting: NFYB CBF-A, HAP3, NF-YB

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31913

Provider

Invitrogen Antibodies

Product name

NFYB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: HeLa, HeLa nuclear extract, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (92%), Pig (99%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.42 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of NFYB was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYB Polyclonal Antibody (Product # PA5-31913) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of NFYB was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYB Polyclonal Antibody (Product # PA5-31913) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NFYB Polyclonal Antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 µg whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 µg of NFYB Polyclonal Antibody (Product # PA5-31913) 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB Polyclonal Antibody (Product # PA5-31913) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of NFYB was performed in paraffin-embedded mouse intestine tissue using NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of NFYB was performed in paraffin-embedded rat stomach tissue using NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:500.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or NFYB Polyclonal Antibody (Product # PA5-31913). The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or NFYB Polyclonal Antibody (Product # PA5-31913). The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NFYB Polyclonal Antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 µg whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 µg of NFYB Polyclonal Antibody (Product # PA5-31913) 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB Polyclonal Antibody (Product # PA5-31913) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.