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LearnPA5-17686
antibody from Invitrogen Antibodies
Targeting: CEBPB
C/EBP-beta, CRP2, IL6DBP, LAP, NFIL6, TCF5
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PA5-17686 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-C/EBP beta (Thr235) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody. This antibody is not cross-reactive with other phosphorylated C/EBPs.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 30 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-Phospho-C/EBP beta (Thr235) Polyclonal Antibody (Product # PA5-17686) and 36, 33 kDa bands corresponding to C/EBP beta (LAP isoforms) and 15 kDa band corresponding to C/EBP beta (LIP isoform) was observed to increase in 3T3-L1 upon LY294002 and Insulin treatment. Also, 15 kDa band corresponding to C/EBP beta (LIP isoform) was increased in Hep G2 upon LY294002 and Insulin treatment. Modified whole cell extracts (1% SDS) (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 treated with LY294002 (50µM for 4hrs) (Lane 2), 3T3-L1 treated with Insulin (1.67µM for 6hrs) (Lane 3), Hep G2 (Lane 4), Hep G2 treated with LY294002 (50µM for 4hrs) (Lane 5), Hep G2 treated with Insulin (1.67µM for 6hrs) (Lane 6) were electrophoresed using Novex® NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
