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LearnNBP1-46179
antibody from Novus Biologicals
Targeting: CEBPB
C/EBP-beta, CRP2, IL6DBP, LAP, NFIL6, TCF5
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-46179 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-46179, RRID:AB_10009411
- Product name
- Rabbit Polyclonal CEBP Beta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CEBP Beta Antibody [NBP1-46179] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. C/EBP beta was also immunoprecipitated by rabbit anti-C/EBP beta antibody NBP1-46180, which recognizes a downstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CEBP Beta Antibody [NBP1-46179] - Detection of Human C/EBP beta by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug), 293T (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody NBP1-46179 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CEBP Beta Antibody [NBP1-46179] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-C/EBP beta used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .
