TA324588

antibody from OriGene

Targeting: CTBP1 BARS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324588

Provider

OriGene

Product name

Rabbit polyclonal CTBP1 Antibody (C-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal CTBP1 Antibody (C-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

CTBP1

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

0.4 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

CTBP1 Antibody (C-term) (Cat. #TA324588) western blot analysis in Hela,HL-60,Jurkat,Raji cell line and mouse brain,rat brain tissue lysates (35ug/lane).This demonstrates the CTBP1 antibody detected the CTBP1 protein (arrow).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of Hela cell stained with CTBP1 Antibody (C-term)(Cat#TA324588). Hela cells were incubated with CTBP1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .CTBP1 immunoreactivity is localized to nucleus significantly.

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of SK-BR-3 cell stained with CTBP1 Antibody (C-term)(Cat#TA324588). SK-BR-3 cells were incubated with CTBP1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .CTBP1 immunoreactivity is localized to nucleus significantly.

Validation comment

IF