ARG55565

antibody from Arigo

Targeting: CTBP1 BARS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG55565

Provider

Arigo

Product name

anti-CtBP1 antibody

Antibody type

Polyclonal

Antigen

KLH-conjugated synthetic peptide around aa. 413-440 (C-terminus) of Human CtBP1.

Description

Purification with Protein A and immunogen peptide.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Storage

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 35 µg of HeLa, HL-60, Jurkat, Raji cell line and mouse brain, rat brain tissue lysates stained with ARG55565 anti-CtBP1 antibody.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: HeLa cell stained with ARG55565 anti-CtBP1 antibody (green) at 1:25 dilution, 1 h at 37°C. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARG55565 anti-CtBP1 antibody. Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.|.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: SK-BR-3 cell stained with ARG55565 anti-CtBP1 antibody (green) at 1:25 dilution, 1 h at 37°C. SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARG55565 anti-CtBP1 antibody. Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.|.