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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF4639 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CtBP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse CtBP1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CtBP2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q13363- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human CtBP1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human CtBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human CtBP1 and CtBP2 (5 ng/lane) were included. A specific band for CtBP1 was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
