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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB7908 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7908, RRID:AB_1707535
- Product name
- DOK1 (phospho S450) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of DOK1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references c-Abl phosphorylates Dok1 to promote filopodia during cell spreading.
IkappaB kinase beta phosphorylates Dok1 serines in response to TNF, IL-1, or gamma radiation.
Tyrosine phosphorylation of p62dok by p210bcr-abl inhibits RasGAP activity.
Woodring PJ, Meisenhelder J, Johnson SA, Zhou GL, Field J, Shah K, Bladt F, Pawson T, Niki M, Pandolfi PP, Wang JY, Hunter T
The Journal of cell biology 2004 May 24;165(4):493-503
The Journal of cell biology 2004 May 24;165(4):493-503
IkappaB kinase beta phosphorylates Dok1 serines in response to TNF, IL-1, or gamma radiation.
Lee S, Andrieu C, Saltel F, Destaing O, Auclair J, Pouchkine V, Michelon J, Salaun B, Kobayashi R, Jurdic P, Kieff ED, Sylla BS
Proceedings of the National Academy of Sciences of the United States of America 2004 Dec 14;101(50):17416-21
Proceedings of the National Academy of Sciences of the United States of America 2004 Dec 14;101(50):17416-21
Tyrosine phosphorylation of p62dok by p210bcr-abl inhibits RasGAP activity.
Kashige N, Carpino N, Kobayashi R
Proceedings of the National Academy of Sciences of the United States of America 2000 Feb 29;97(5):2093-8
Proceedings of the National Academy of Sciences of the United States of America 2000 Feb 29;97(5):2093-8
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot of Jurkat cells stimulated with calyculin A (100 nM, 30 min) (lane 1) followed by lambda phosphatase (lane 2) treatment. The blots were probed with DOK1 (phospho S450) polyclonal antibody (Cat # PAB7908).
